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. 2017 Jan 1;130(1):269–277. doi: 10.1242/jcs.184341

Fig. 4.

Fig. 4.

Crossing epithelial Gal4 lines to UAS lines that provide tools for developmental studies. (A,B) Cells can be fluorescently labeled and tracked over time with the periderm Gal4 line (zc1044A) driving expression of photo-convertible protein Kaede, Tg(UAS-E1b:Kaede)s1999t. Expression of Kaede is detected as green fluorescence before exposure to blue (UV) light (A), and red (shown here as magenta) after exposure (B). (C-F) The labeled epithelial cells can be readily ablated using the nitroreductase–metronidazole system, Tg(UAS-E1b:nfsB-mCherry). (C-D) Representative bright-field images of wild-type (C) and zc1036A (D) 48 hpf embryos after 24 h treatment with MTZ to ablate the cells. Arrowhead in D′ denotes defects in the developing median fin epidermis. (E-F) The MTZ-exposed zc1036A cells undergo apoptosis (activated caspase-3-positive cells in green) and cause defects in the developing embryo. C-D represent data from a single experiment performed in triplicate. Scale bars: 25 μm in A-B, 250 μm in C-D, 50 μm in E-F.