Spatial and temporal control of Gal4-driven gene expression. (A) The photo-cleavable morpholino directed against gal4 was injected in Et(Gal4-VP16)zc1044A;Tg(UAS-E1b:nsfB-mCherry) transgenic line and embryos were exposed to UV light at 28-32 hpf. (B-H) Representative bright-field (B,D,F) and fluorescent (C,E,G,H) images 48 h after exposure to UV, at 3 dpf. Box in G denotes area of magnification for H. B-H represent data from three independent experiments. Scale bars: 250 μm in B-G, 50 μm in H.