Fig. 5.
The ZO-1 U5-GuK domain is necessary for tight junction assembly in 2D cultures. ZO-1 KD cells expressing EGFP–ZO-1 mutants were subjected to the Ca2+-switch assay and stained for either PATJ and E-cadherin or occludin at the indicated time points. Full monolayer thickness maximal projections are shown and are representative of three independent experiments per condition. Yellow arrowheads denote protein recruitment to tight junctions, and red arrowheads indicate cells with high expression levels of EGFP–ZO-1ΔU5-GuK. Scale bars: 10 µm. Additional images are shown in Fig. S3D.