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. 2016 Jun 30;30(4):576–584. doi: 10.5713/ajas.16.0207

Figure 1.

Figure 1

Codon optimization and expression vector construction of pBD-2/cecropin P1 fusion gene. (A) Sequence comparison between wild-type and codon optimized pBD-2/cecropin P1 fusion gene. Opt, codon-optimized; WT, wild-type; AA, animo acids; AP leader, signal peptide of alkaline protease; ASASA, a linker; DDDDK, enterokinase site; His-tag, 6-histidine tag. (B) Prokaryotic expression vector of pBD-2/cecropin P1 fusion gene. The fusion gene (465 bp) was controlled by Lac promoter. BD-CP1, pBD-2/cecropin P1 fusion gene. (C) Restriction analysis of the pMK4-BD/CP1/His plasmid. M, DL 2000DNA Marker; Lane 1, the pMK4-BD/CP/His plasmid; Lane 2, digested fragments of the pMK4-BD/CP/His plasmid by Nde I and BamH I; Lane 3, digested fragments by BamH I and EcoR I.