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The effect of CLL cells on ILC cytokine production. PBMCs from HCs (n=3) depleted from monocytes, T and B cells were cocultured with CLL cells in a 1:10 ratio or medium alone overnight. (A) Brefeldin A was added during the final 4 hours of culture. Percentage of ILCs producing cytokines after coculture. (B) Cells were stimulated with PMA/ionomycin in the presence of Brefeldin A during the final 4 hours of culture. The percentage of ILCs producing cytokines after coculture.
