Table 1.
Identification of proteins differentially expressed between WT and spl5 mutant
| Function type | Spot id | Homologous protein | Score | Source | Accession | Coverage (%) | pI | MM (kDa) | Change fold | mRNA level |
|---|---|---|---|---|---|---|---|---|---|---|
| mRNA splicing | 2 | Thioredoxin-like protein 4B | 71 | O. sativa | gi|125576924 | 25 | 6.4 | 23.6 | −3.3 | - |
| Amino-acid metabolism | 4 | Alanine aminotransferase | 187 | O. sativa | gi|115470235 | 31 | 8 | 54.0 | −2.3 | - |
| 6 | Aspartate aminotransferase | 84 | O. sativa | gi|125541475 | 15 | 6.5 | 50.6 | 2.0 | up | |
| 12 | Cysteine synthase | 86 | O. sativa | gi|115489664 | 29 | 5.3 | 33.9 | −3.9 | - | |
| 5 | S-adenosylmethionine synthetase | 93 | O. sativa | gi|100801534 | 27 | 6.5 | 43.0 | 2.6 | up | |
| Photosynthesis | 3 | Rubisco large subunit | 130 | O. sativa | gi|115468792 | 33 | 8.5 | 48.4 | −2.7 | down |
| 9 | Rubisco activase | 74 | O. sativa | gi|1778414 | 28 | 5.4 | 48.1 | −2.0 | - | |
| 10 | Rubisco activase, chloroplast precursor | 174 | O. sativa | gi|108864712 | 44 | 5.1 | 36.7 | −3.1 | - | |
| Glycolysis | 8 | Glyceraldehyde-3-phosphate dehydrogenase | 78 | O. sativa | gi|115459078 | 24 | 7.3 | 36.9 | 3.1 | up |
| ROS metabolism | 11 | Glutathione S-transferase 14 | 88 | O. sativa | gi|46276327 | 33 | 6.5 | 30.8 | 2.1 | up |
| 13 | Ascorbate peroxidase 7 | 264 | O. sativa | gi|116310282 | 47 | 6.9 | 38.2 | 8.1 | up | |
| Defense-related | 14 | Chitinase Chia2a | 81 | O. sativa | gi|115483206 | 27 | 5.4 | 27.9 | 3.5 | up |
| Others | 1 | Retrotransposon Ty3-gypsy subclass | 68 | O. sativa | gi|77556153 | 16 | 4.7 | 29.1 | 3.0 | down |
| 7 | Nad-dependent formate dehydrogenase | 164 | O. sativa | gi|4760553 | 40 | 7.2 | 41.5 | −2.2 | - |
Spot ID refers to the spot identity as given in Figure 2; Accession, protein accession in GenBank (http://www.ncbi.nlm.nih.gov/); Coverage %, the percentage of sequence coverage; pI, experimental isoelectric points; MM, experimental molecular masses; Change fold, the expression change fold of protein level in spl5 mutant compared to WT; mRNA level, the expression change of mRNA level in spl5 mutant compared to WT.