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Age of rat pups used for RGC cultures and length of time in culture did not affect the velocity of phosphatidylserine externalization. A, There was no significant difference in the velocity of annexin B12-IANBD signal among RGCs harvested from pups ranging from P2 to P7. B, Length of time in culture for RGCs used in axotomy experiments ranged between 3 and 8 d. There was no significant effect on the velocity of phosphatidylserine externalization.
