Table 2.
Primer sequences for T7 endonuclease assay and quantitative RT-PCR.
| Genes | Sequences | Amplicon size |
|---|---|---|
| Primer for T7 EI assay | ||
| MYOG gDNA_F | 5′-GGCCGCCCAGCTAGGAGTAATTGA-3′ | 786 |
| MYOG exon1_R | 5′-CGCTCGATGTACTGGATGGCACTG-3′ | |
| Primer for RT-qPCR | ||
| RPL13A_F | 5′-CCCTGGAGGAGAAGAGGAAA-3′ | 91 |
| RPL13A_R | 5′-ACGTTCTTCTCGGCCTGTTT-3′ | |
| MYOG_F | 5′-GCTCAGCTCCCTCAACCA-3′ | 94 |
| MYOG_R | 5′-GCTGTGAGAGCTGCATTCG-3′ | |
| MYOD1_F | 5′-GCACTACAGCGGCGACTCC-3′ | 118 |
| MYOD1_R | 5′-GTAGGCGCCTTCGTAGCAG-3′ | |
| Endo-MYOD1_F | 5′-CACTCCGGTCCCAAATGTAG-3′ | 180 |
| Endo-MYOD1_R | 5′-TTCCCTGTAGCACCACACAC-3′ | |
| MRF4_F | 5′-GGCCAAGTGTTTCCGATCAT-3′ | 89 |
| MRF4_R | 5′-AAGGCTACTCGAGGCTGACG-3′ | |