Table 2.

Plasmids used in this study

Plasmid	Description
pRW0226117	YIp128-GAL-PDS1-mDB-HA
pRW0220112	YIp204-CDC20p-8Myc-CDC20
pRW0225111	YIp204-CDC20p-8Myc-CDC20ΔC
pRW0801103	pAS2-CDC20ΔC
pRW1218101	pAS2-CDC20ΔC-mDB1
pRW1207101	pAS2-CDC20ΔC-mDB2
pRW0110115	pAS2-CDC20ΔC-mDB3
pRW1207103	pAS2-CDC20ΔC-mDB4
pRW0319116	pAS2-CDC20ΔC-mDB4-KA
pRW0319117	pAS2-CDC20ΔC-mDB4-KL
pRW0716111	pAS2-CDC20Δ(C+IR)
pRW0319114	PACTII-MAD3
pRW0504101	PACTII-MAD2
pRW0504103	PACTII-HSL1(667–872)
pRW0331111	pRS303-MAD3Δ
pRW0226111	YIp204-CDC20p-8Myc-CDC20-mDB1
pRW0226113	YIp204-CDC20p-8Myc-CDC20-mDB2
pRW0226115	YIp204-CDC20p-8Myc-CDC20-mDB3
pRW0704111	YIp204-CDC20p-8Myc-CDC20ΔC-mDB1
pRW0704113	YIp204-CDC20p-8Myc-CDC20ΔC-mDB2
pRW0704115	YIp204-CDC20p-8Myc-CDC20ΔC-mDB3
pRW0510111	YIp204-CDC20p-8Myc-CDC20Δ(C+IR)
pRW1229114	YIp204-CDC20p-lacZ
pRW1025135	YCp22-CDC20p-lacZ
pRW0609113	pRS303-NDD1(781 bp-end)-3×Flag
pRW1125115	pRS303-MCM1(301 bp-end)-3×Flag
pRW0802111	pRS303-FKH1(301 bp-end)-3×Flag
pRW0504142	pRS303-FKH2(1291 bp-end)-3×Flag
pRW0118121	pRS303-YoX1(301 bp-end)-3×Flag
pRW0402161	pRS313-MET3p-CLB2-3×Flag
pRW0402162	pRS313-MET3p-CLB5-3×Flag
pRW1212163	pRS303-MET3p-8Myc-CLB5(1-650bp)
pRW1221161	YIp211-MET3p-8Myc-CLB2(1-800bp)