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. 2017 Mar 14;2:18. [Version 1] doi: 10.12688/wellcomeopenres.11005.1

Figure 5. Knocksideways (KS) of Plasmodium proteins.

Figure 5.

( A– I) Images of KS parasites expressing FKBP::GFP tagged protein, as indicated, showing upper panel minus (-) and lower panel plus 200 nM rapamycin (RAP) (+). Images were taken on a Deltavision core microscope and deconvoluted using softWoRx software. Showing mCherry (mCH), GFP, Hoechst ( H) and DIC images. Scale bars are 5 µm. Parasites in ( E) were stained with the RBC membrane marker Ter119 instead of Hoechst and parasites in ( F) show a maximum projection of the deconvoluted Z stack. Graphs show growth curves for each KS line minus (dark line) and plus (red line) Rap treatment at 1 mg/kg by IP injection usually on days 2 and 3 (+), except for ( C and H) when Rap was additionally given on day 0 and 1. Graphs show % parasitaemia (y axis) measured by flow cytometry gated on mCherry and Hoechst positive parasites over 5 days (x axis). Mean +/- SD of 2–4 mice per group. The possible growth discrepancy in ( D) was not significant. ( J) GAP50 KS ookinetes after RAP treatment for 24 hours. Plot shows the number of midgut oocyst counts on day 10–14 after in vivo, GAP50 KS induction one hour prior to transmission feed for three independent transmissions minus (-) and plus (+) RAP.