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. Author manuscript; available in PMC: 2017 Apr 18.
Published in final edited form as: Biochimie. 2017 Jan 3;134:35–50. doi: 10.1016/j.biochi.2016.12.016

Fig. 3. UCP1 is a monomer binding GDP with a 1:1 stoichiometry.

Fig. 3

(A) Assessment of the molecular mass of UCP1 in 10MNG/cardiolipin by size exclusion chromatography [36]. Inset shows the mass composition of the major peak. (B) The apparent molecular mass of native UCP1 and ADP/ATP carrier in blue native gels [97]. Mitochondrial membrane extracts from lamb brown adipose tissue (UCP1, left), rat liver (ADP/ATP carrier, middle) and yeast mitochondria (monomer and covalently linked dimer of the ADP/ATP carrier AAC3, right) were prepared in 1% dodecylmaltoside and separated in 6–18% (w/v) polyacrylamide gels. (C) Isothermal titration calorimetry of purified UCP1 with GDP [36]. The enthalpy changes associated with the titration of GDP into UCP1 at 4 °C (top), and corresponding isotherms fitted to a one-site binding model with ∆H, Kd, and stoichiometry as fitting parameters (bottom).