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. Author manuscript; available in PMC: 2017 Apr 18.
Published in final edited form as: Cell Rep. 2017 Apr 4;19(1):188–202. doi: 10.1016/j.celrep.2017.03.030

Figure 3. Keap1 modulates p62 ubiquitination.

Figure 3

(A) Anti-HA and anti-Flag immunoblots of HA-immunoprecipitated lysates from Keap1−/−MEFs expressing HA-p62-WT, Flag-Ub and myc-Keap1. (B) Anti-HA and anti-Flag immunoblots of HA-immunoprecipitated lysates from p62−/−MEFs expressing HA-p62, Flag-Ub, V5-cul3 and myc-Keap1-WT or myc-Keap1-ΔBTB. (C) Anti-HA, anti-Flag, and LC3 immunoblots of HA-immunoprecipitated lysates from Keap1−/−MEFs expressing Flag-Ub, HA-p62-WT, HA-p62-T350A or HA-p62-ΔUBA with or without myc-Keap1. (D) anti-p62 immunoblot of nickel resin purified lysates from U20S cells expressing His-Ub and treated with DMSO or MLN4924. (E) Anti-p62 immunoblot of lysates from U20S cells with or without Myc-Keap1/V5-Cul3 expression or treated with MLN4924. Anti-GAPDH serves as loading control for blots.