Figure 1.

Synopsis of sequence analysis of FLT3-ITD in AML patients. A. Number of patients with single FLT3-ITD and patients with two or more internal tandem duplications. B. Principle of cloning, colony screening, and sequence analysis to determine the exact sequence of each individual FLT3-ITD. C. Illustration of all 57 identified FLT3-ITDs indicating starting point, length and end point of each of each ITD according to the amino acid sequence of FLT3. The x-axis indicates the corresponding codons of FLT3 protein sequence. All applied methods are comprehensively described in Online Supplementary Material. D. Sequence analyses of FLT3-ITDs demonstrating its localization and length as well as the proportion of patients presenting a duplication of distinct peptide motifs, e.g., YVDFREY (position 591 to 597), the STAT5 binding site (Y589/Y591) or “Y-X-Y” motifs in general. IQR, interquartile range; aa, amino acid.