Table 1. Nucleotide sequences of the forward and reverse primers used for qPCR.
| Gene | accession | forward primer | reverse primer | amplicon |
|---|---|---|---|---|
| Name | number | Sequence | sequence | length |
| elf1a | AY422992 | CTGGAGGCCAGCTCAAACAT | TCAAGAAGAGTAGTACCGCTAGCATTAC | 85 |
| rpl13 | NM_212784 | TCTGGAGGACTGTAAGAGGTATGC | AGACGCACAATCTTGAGAGCAG | 147 |
| crf | BC164878 | CGAGACATCCCAGTATCCAAAAAG | TCCAACAGACGCTGCGTTAA | 59 |
| crf-bp | NM_001003459 | ACAATGATCTCAAGAGGTCCAT | CCACCAAGAAGCTCAACAAA | 66 |
| mr | NM_001100403 | CTTCCAGGTTTCCGCAGTCTAC | GGAGGAGAGACACATCCAGGAAT | 74 |
| grα | EF436284.1 | ACTCCATGCACGACTTGGTG | GCATTTCGGGAAACTCCACG | 90 |
| grβ | EF436285.1 | GATGAACTACGAATGTCTTA | GCAACAGACAGCCAGACAGCTCACT | 210 |
| bdnf | NM_131595 | AGAGCGGACGAATATCGCAG | GTTGGAACTTTACTGTCCAGTCG | 110 |
| pcna | AF140608.1 | AAGGAGGATGAAGCGGTAACAAT | GTCTTGGACAGAGGAGTGGC | 104 |
| neurod1 | AF036148.1 | TCCCTACTCCTACCAGACGC | CAGTCTGTGAGGGTGGTGTC | 135 |
| igf1 | NM_131825.2 | GGCGCCTCGAGATGTATTGT | CCTCGGCTCGAGTTCTTCTG | 160 |
| gh1 | NM_001020492.2 | GCATCAGCGTGCTCATCAAG | TGAGACTGGTCTCCCCTACG | 114 |
| cart4 | NM_001082932.1 | GCTGAGGCACTCGATGAACT | GAAGAAAGTGTTGCAGGCGG | 174 |
| socs3a | NM_199950.1 | GCAGGAAGACTGTGAACGGA | CAGTGGCTGGACAACTCCAT | 192 |
| irg1l | NM_001077607.1 | TTTCAAGCGCTTTCCTGCAC | GAGAGTGGCACCCTAAGCAG | 141 |
| mpeg1.1 | NM_212737.1 | CCACAGAAAGTGAGCGGAGT | GGACTTGAACCCGTGCTGTA | 196 |
| mpeg1.2 | NM_001020586.1 | CACGGCGGGGCTTTATTCTA | GAGGTCAGGGAATACGGCTG | 135 |
Genes: stress-axis genes: corticotropin-releasing factor (crf), crf-binding protein (crf-bp), mineralocorticoid receptor (mr), glucocorticoid receptor α (grα) and β (grβ); ‘(neuro)development related’ genes: proliferating cell nuclear antigen (pcna); brain derived neurotrophic factor (bdnf); neuronal differentiation factor 1 (neurod1); insulin growth-factor 1 (igf1); growth hormone 1 (gh1); cocaine- and amphetamine-regulated transcript 4 (cart4); ‘(innate) immune system related’ genes: suppressor of cytokine signalling 3a (socs3a), immune-responsive gene 1-like (irg1l), macrophage-expressed gene 1 (mpeg1.1; also called mpeg1), macrophage-expressed gene 1.2 (mpeg1.2); reference genes: elongation factor 1α (elf1α); ribosomal protein L13 (rpl13).