Table 2. Mean (± SEM) relative expression normalised to elf1a/rpl13 for genes of interest in 5 dpf larvae of AB and TL.
Gene | AB (n = 10) | TL (n = 10) | Statistics |
---|---|---|---|
crf | 2.75 ± 0.25 | 2.00 ± 0.16 | t = 2.566; df = 18; p ≤ 0.02 |
crf-bp | 1.60 ± 0.09 | 1.07 ± 0.07 | t = 4.546; df = 18; p ≤ 0.001 |
mr | 3.22 ± 0.33 | 2.67 ± 0.12 | |
gr-alpha | 0.66 ± 0.04 | 1.30 ± 0.11 | t = -5.368; df = 18; p ≤ 0.001 |
gr-beta | 0.78 ± 0.09 | 0.10 ± 0.03 | t = 7.508; df = 18; p ≤ 0.001 |
bdnf | 1.70 ± 0.07 | 0.58 ± 0.09 | t = 9.735; df = 18; p ≤ 0.001 |
pcna | 0.49 ± 0.03 | 0.14 ± 0.01 | t = 11.689; df = 18; p ≤ 0.001 |
neurod1 | 1.33 ± 0.11 | 0.77 ± 0.09 | t = 3.899; df = 18; p ≤ 0.001 |
gh1 | 0.10 ± 0.01 | 0.09 ± 0.01 | |
cart4 | 0.41 ± 0.04 | 0.12 ± 0.03 | t = 5.457; df = 18; p ≤ 0.001 |
igf1 | 1.89 ± 0.21 | 0.83 ± 0.07 | t = 4.693; df = 18; p ≤ 0.001 |
socs3a | 0.07 ± 0.004 | 0.04 ± 0.006 (n = 9) | t = 4.175; df = 17; p ≤ 0.001 |
mpeg1.1 | 0.13 ± 0.02 (n = 9) | 0.08 ± 0.008 | t = 2.281; df = 17; p ≤ 0.04 |
mpeg1.2 | 0.50 ± 0.07 | 0.05 ± 0.01 | t = 5.996; df = 18; p ≤ 0.001 |
irg1l | 0.24 ± 0.05 | 0.03 ± 0.008 (n = 7) | t = 3.807; df = 15; p ≤ 0.002 |
Unless otherwise indicated n = 10 samples per strain (2 larvae/sample); Reasons for less than 10 samples: poor signal (AB: mpeg1.1; TL: irg1l (n = 2)) or outliers (Grubb’s test, p≤0.01; TL: socs3a; TL: irg1l); Statistics between AB and TL are shown (Student’s t-test).