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. 2017 Apr 18;12(4):e0175420. doi: 10.1371/journal.pone.0175420

Table 2. Mean (± SEM) relative expression normalised to elf1a/rpl13 for genes of interest in 5 dpf larvae of AB and TL.

Gene AB (n = 10) TL (n = 10) Statistics
crf 2.75 ± 0.25 2.00 ± 0.16 t = 2.566; df = 18; p ≤ 0.02
crf-bp 1.60 ± 0.09 1.07 ± 0.07 t = 4.546; df = 18; p ≤ 0.001
mr 3.22 ± 0.33 2.67 ± 0.12
gr-alpha 0.66 ± 0.04 1.30 ± 0.11 t = -5.368; df = 18; p ≤ 0.001
gr-beta 0.78 ± 0.09 0.10 ± 0.03 t = 7.508; df = 18; p ≤ 0.001
bdnf 1.70 ± 0.07 0.58 ± 0.09 t = 9.735; df = 18; p ≤ 0.001
pcna 0.49 ± 0.03 0.14 ± 0.01 t = 11.689; df = 18; p ≤ 0.001
neurod1 1.33 ± 0.11 0.77 ± 0.09 t = 3.899; df = 18; p ≤ 0.001
gh1 0.10 ± 0.01 0.09 ± 0.01
cart4 0.41 ± 0.04 0.12 ± 0.03 t = 5.457; df = 18; p ≤ 0.001
igf1 1.89 ± 0.21 0.83 ± 0.07 t = 4.693; df = 18; p ≤ 0.001
socs3a 0.07 ± 0.004 0.04 ± 0.006 (n = 9) t = 4.175; df = 17; p ≤ 0.001
mpeg1.1 0.13 ± 0.02 (n = 9) 0.08 ± 0.008 t = 2.281; df = 17; p ≤ 0.04
mpeg1.2 0.50 ± 0.07 0.05 ± 0.01 t = 5.996; df = 18; p ≤ 0.001
irg1l 0.24 ± 0.05 0.03 ± 0.008 (n = 7) t = 3.807; df = 15; p ≤ 0.002

Unless otherwise indicated n = 10 samples per strain (2 larvae/sample); Reasons for less than 10 samples: poor signal (AB: mpeg1.1; TL: irg1l (n = 2)) or outliers (Grubb’s test, p≤0.01; TL: socs3a; TL: irg1l); Statistics between AB and TL are shown (Student’s t-test).