(A) Left, schematic diagram showing C4-2 cell clones expressing PSA eRNA in which the TAR-L motif or a control region was deleted by TALENs. Right, expression of PSA eRNA and mRNA in C4-2 TAR-L deletion stable cells measured by RT-qPCR. Data shown as means±SD (n=3). *** P < 0.001 comparing to control Δ1.
(B) ChIP-qPCR analysis of Pol II Ser2p at the PSA gene locus in C4-2 TALEN modified cells. Data shown as means±SD (n=3). * P < 0.05 comparing to control Δ1.
(C) ChIP-qPCR analysis of HEXIM1 binding at the PSA promoter. ChIP DNA was analyzed by real-time PCR. Data shown as means±SD (n=3). ** P < 0.01 comparing to control Δ1.
(D) ChIP-qPCR analysis of Pol II-Ser2p at the trans target loci VEGFA and SLC45A3 in C4-2 TALEN modified cells. Data shown as means±SD (n=3). * P < 0.01 comparing to control Δ1.
(E and F) Viability of mock or ASO-transfected, or control or TALEN-modified C4-2 cells was measured by MTS assay. Data shown as means±SD (n=6). ** P < 0.01; *** P < 0.001 comparing to control 1.
(G) A hypothetic model of PSA eRNA-mediated cis and trans activation of the P-TEFb complex and gene transcription.