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(A) 10% SDS-PAGE of pure recombinant MsPrsA: molecular weight markers are on the left (M) and the pure enzyme on the right showing a molecular weight of about 37 KDa. (B) Elution profile of MsPrsA on a HiPrep 16/60 Sephacryl 200 High Resolution pre-packed column. In the inset, the arrow indicates the MsPrsA elution volume on the experimentally determined calibration curve.
