Table 3. Protein yield from 2 L culture.
Operation Step | Total Protein (mg) | Total Activity (U) | Specific Activity (U/mg) | Fold Purification | Yield (%) |
---|---|---|---|---|---|
Crude Lysate | 226.0 | 723.2 | 3.2 | 1 | 100 |
Ni-NTA | 20.0 | 7.2 | 0.4 | 0.11 | 0.99 |
S-200 | 12.8 | 17.8 | 1.4 | 0.44 | 2.47 |
Final MsPrsA | 9.1 | 12.7 | 1.4 | 0.44 | 1.76 |