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. 2017 Apr 18;12(4):e0175815. doi: 10.1371/journal.pone.0175815

Table 3. Protein yield from 2 L culture.

Operation Step Total Protein (mg) Total Activity (U) Specific Activity (U/mg) Fold Purification Yield (%)
Crude Lysate 226.0 723.2 3.2 1 100
Ni-NTA 20.0 7.2 0.4 0.11 0.99
S-200 12.8 17.8 1.4 0.44 2.47
Final MsPrsA 9.1 12.7 1.4 0.44 1.76