Fig 2. Chaetocin induces apoptosis in human melanoma cells.

Apoptosis was detected by Annexin V-FITC/PI using flow cytometry. (A) The representative plots of flow cytometry for chaetocin-induced apoptosis in Sk-Mel-28 and A375 cells which were treated with 0, 5 and 10 μM chaetocin for 24 h. (B) The cells were treated with indicated concentrations of chaetocin for 24 h, and demonstrated an increasing apoptotic rate in a dose-dependent manner. (C-D) Sk-Mel-28 (C) and A375 (D) cells were treated without or with 10 μM chaetocin for 24, 48 and 72 h, and apoptotic cells were determined with flow cytometry. Data represented as means ± SEM (n = 3). *P< 0.05 and **P< 0.01, compared with the controls.