Table 2. Sensitivity and specificity of Hcp1-ELISA, OPS-ELISA and Hcp1/OPS-ELISA.
The assay values were calculated from Thai patients who had melioidosis, tuberculosis, scrub typhus, leptospirosis and Thai healthy donors. The cut-off values with Thai healthy donors as controls were used at specificity of 95% for each assay.
| ELISA | Cut-off OD | % Sensitivity (CI) | % Specificity (CI) | ||||
|---|---|---|---|---|---|---|---|
| Thai melioidosis patients (N = 141) |
Thai healthy donors (N = 188) |
U.S. healthy donors (N = 90) |
Tuberculosis patients (N = 20) |
Scrub typhus patients (N = 50) |
Leptospirosis patients (N = 50) |
||
| Hcp1 | 1.165 | 83.0 (75.7–88.8) | 96.3 (92.5–98.5) | 95.6 (89.0–98.8) | 100 (83.2–100) | 98.0 (89.4–100) | 100 (92.9–100) |
| OPS | 0.875 | 71.6 (63.4–78.9) | 95.7 (91.1–97.8) | 96.7 (90.6–99.3) | 100 (83.2–100) | 94.0 (83.5–98.8) | 98.0 (89.4–100) |
| Hcp1/OPS | 1.125 | 81.6 (74.2–87.6) | 95.2 (91.1–97.8) | 98.9 (94.0–100) | 100 (83.2–100) | 88.0 (75.7–95.5) | 96.0 (86.3–99.5) |