Table 4. Five proteins that showed the highest positive uniparental heterosis in maize F1 hybrids 2023×CE704 or CE704×2023 under conditions of normal water supply.

Protein	Matching sequence (EST/protein)	23×04/23	23×04/04	04×23/23	04×23/04
Ranked according to the difference between 2023×CE704 and 2023
Glycine-rich protein1	gi|219888685	10,1	-1,7	9,9	-1,8
Peroxidase	gi|257738102	4,9	-2,1	5,0	-1,9
Protein binding protein	gi|238011090	3,3	4,1	1,7	2,1
Peroxiredoxin bcp	gi|226507110	3,3	1,0	2,9	-1,1
Hypothetical protein	gi|195655323	3,2	-2,3	4,4	-1,9
Ranked according to the difference between 2023×CE704 and CE704
Glycine-rich RNA-binding protein 2	gi|257743787	-1,8	48,8	-1,9	53,0
ABA-stress-ripening inducible-like protein	gi|269913328	-1,2	11,0	-1,6	9,4
PSI subunit—PsaL	gi|195613284	-2,1	7,8	-2,4	7,2
Phosphoenolpyruvate carboxylase	gi|27764449	1,1	5,2	-3,7	1,3
Dehydrin 13	gi|195625830	2,6	4,4	5,2	6,1
Ranked according to the difference between CE704×2023 and 2023
Glycine-rich protein1	gi|219888685	10,1	-1,7	9,9	-1,8
Dehydrin 13	gi|195625830	2,6	4,4	5,2	6,1
Peroxidase	gi|257738102	4,9	-2,1	5,0	-1,9
Hypothetical protein	gi|195655323	3,2	-2,3	4,4	-1,9
Pro-resilin	gi|195620516	2,2	-1,7	4,2	1,5
Ranked according to the difference between CE704×2023 and CE704
Glycine-rich RNA-binding protein 2	gi|257743787	-1,8	48,8	-1,9	53,0
ABA-stress-ripening inducible-like protein	gi|269913328	-1,2	11,0	-1,6	9,4
PSI subunit—PsaL	gi|195613284	-2,1	7,8	-2,4	7,2
Dehydrin 13	gi|195625830	2,6	4,4	5,2	6,1
Retrotransposon protein	gi|226510042	-1,5	1,6	1,5	3,0

The numbers in the individual columns represent the n-fold difference in the protein level between the respective F1 hybrid and its parental inbred line, as derived from the iTRAQ analysis ratios. These ratios are shown as inverse values with a negative sign in case of the higher protein level in the respective inbred line. 23×04 represents the 2023×CE704, 04×23 represents the CE704×2023, 23 represents the 2023 and 04 represents the CE704.