Table 5. Five proteins that showed the highest negative uniparental heterosis in maize F1 hybrids 2023×CE704 or CE704×2023 under conditions of normal water supply.
| Protein | Matching sequence (EST/protein) | 23×04/23 | 23×04/04 | 04×23/23 | 04×23/04 |
|---|---|---|---|---|---|
| Ranked according to the difference between 2023×CE704 and 2023 | |||||
| Heat shock protein (17.4 kDa, class I) | gi|296512733 | -5,5 | -5,4 | -1,3 | -1,1 |
| Rubisco LSU-binding protein subunit alpha (GroEL) | gi|257734906 | -2,3 | 1,1 | -2,4 | 1,1 |
| PSI subunit—PsaL | gi|195613284 | -2,1 | 7,8 | -2,4 | 7,2 |
| Actin-7 | gi|238011086 | -2,0 | -4,5 | 1,5 | -1,6 |
| Heat shock protein (70 kDa, 4-like) | gi|308081377 | -2,0 | 1,2 | -1,0 | 2,2 |
| Ranked according to the difference between 2023×CE704 and CE704 | |||||
| Heat shock protein (17.4 kDa, class I) | gi|296512733 | -5,5 | -5,4 | -1,3 | -1,1 |
| Actin-7 | gi|238011086 | -2,0 | -4,5 | 1,5 | -1,6 |
| Plastid-lipid-associated protein 2 (PAP/fibrillin family) | gi|226498852 | -1,9 | -2,9 | 1,2 | -1,4 |
| Peroxidase 54 | gi|25811927 | -1,1 | -2,7 | 1,9 | -1,1 |
| Glucose-6-phosphate isomerase | gi|293333684 | -1,1 | -2,5 | 1,3 | -1,6 |
| Ranked according to the difference between CE704×2023 and 2023 | |||||
| Phosphoenolpyruvate carboxylase | gi|27764449 | 1,1 | 5,2 | -3,7 | 1,3 |
| Chlorophyll a/b binding protein (6A) | gi|226503327 | 1,2 | 2,4 | -3,5 | -1,8 |
| Late embryogenesis abundant protein, group 3 | gi|7387829 | 1,6 | 1,9 | -3,2 | -2,8 |
| Chlorophyll a/b binding protein (CP24) | gi|226531392 | 1,1 | 2,2 | -3,0 | -1,5 |
| 6-phosphogluconolactonase | gi|226493090 | -1,3 | 1,1 | -2,6 | -1,8 |
| Ranked according to the difference between CE704×2023 and CE704 | |||||
| Sucrose synthase 3 | gi|22121990 | 1,7 | -1,8 | -1,0 | -4,1 |
| Late embryogenesis abundant protein, group 3 | gi|7387829 | 1,6 | 1,9 | -3,2 | -2,8 |
| Fructose-bisphosphate aldolase (cytoplasmic isozyme) | gi|194703646 | -1,0 | -1,4 | -1,9 | -2,7 |
| Catalase 3 isoform 1 | gi|257675731 | -1,0 | -1,8 | -1,5 | -2,4 |
| ATPase 2 isoform 1 (plasma membrane) | gi|219888401 | -1,1 | -1,5 | -1,6 | -2,2 |
The numbers in the individual columns represent the n-fold difference in the protein level between the respective F1 hybrid and its parental inbred line, as derived from the iTRAQ analysis ratios. These ratios are shown as inverse values with a negative sign in case of the higher protein level in the respective inbred line. 23×04 represents the 2023×CE704, 04×23 represents the CE704×2023, 23 represents the 2023 and 04 represents the CE704.