Table 6. Five proteins that showed the highest positive uniparental heterosis in maize F1 hybrids 2023×CE704 or CE704×2023 after 10 days of drought.
| Protein | Matching sequence (EST/protein) | 23×04/23 | 23×04/04 | 04×23/23 | 04×23/04 |
|---|---|---|---|---|---|
| Ranked according to the difference between 2023×CE704 and 2023 | |||||
| Calvin cycle protein CP12-1 | gi|226493683 | 18,4 | 2,3 | 9,3 | -1,9 |
| Triosephosphate isomerase, cytosolic | gi|195658525 | 5,0 | -3,2 | 6,1 | -2,2 |
| PSII subunit—PsbO | gi|224028817 | 4,8 | 1,3 | 4,7 | 1,1 |
| Pro-resilin | gi|195620516 | 4,4 | 1,2 | 3,7 | -1,1 |
| GroEL-like type I chaperonin | gi|257720020 | 4,1 | -1,7 | 5,6 | -1,2 |
| Ranked according to the difference between 2023×CE704 and CE704 | |||||
| Glycine-rich RNA-binding protein 2 | gi|257743787 | -2,9 | 31,6 | -1,5 | 40,6 |
| ABA-stress-ripening inducible-like protein | gi|269913328 | -1,6 | 26,8 | -1,9 | 22,5 |
| PSI subunit—PsaL | gi|195613284 | -1,9 | 10,3 | -2,4 | 8,1 |
| Dehydrin 13 | gi|195625830 | -1,2 | 4,6 | -1,9 | 3,2 |
| Late embryogenesis abundant protein, group 3 | gi|7387829 | 1,1 | 4,0 | -2,1 | 1,6 |
| Ranked according to the difference between CE704×2023 and 2023 | |||||
| Glycine-rich protein1 | gi|219888685 | 2,2 | -8,4 | 9,7 | -2,5 |
| Calvin cycle protein CP12-1 | gi|226493683 | 18,4 | 2,3 | 9,3 | -1,9 |
| Pyruvate,orthophosphate dikinase | gi|257659143 | 3,7 | -2,3 | 9,2 | -1,0 |
| ATP synthase β subunit (chloroplastic) | gi|902229 | 1,1 | -5,2 | 7,4 | 1,1 |
| Glyceraldehyde-3-phosphate dehydrogenase B | gi|238011684 | 3,2 | -3,1 | 6,8 | -1,6 |
| Ranked according to the difference between CE704×2023 and CE704 | |||||
| Glycine-rich RNA-binding protein 2 | gi|257743787 | -2,9 | 31,6 | -1,5 | 40,6 |
| ABA-stress-ripening inducible-like protein | gi|269913328 | -1,6 | 26,8 | -1,9 | 22,5 |
| PSI subunit—PsaL | gi|195613284 | -1,9 | 10,3 | -2,4 | 8,1 |
| Dehydrin 13 | gi|195625830 | -1,2 | 4,6 | -1,9 | 3,2 |
| Heat shock protein (16.9 kDa, class I) | gi|296512550 | 1,1 | 3,7 | -1,2 | 2,7 |
The numbers in the individual columns represent the n-fold difference in the protein level between the respective F1 hybrid and its parental inbred line, as derived from the iTRAQ analysis ratios. These ratios are shown as inverse values with a negative sign in case of the higher protein level in the respective inbred line. 23×04 represents the 2023×CE704, 04×23 represents the CE704×2023, 23 represents the 2023 and 04 represents the CE704.