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. 2017 Apr 12;11:45–53. doi: 10.2147/BTT.S127650

Figure 3.

Figure 3

Knockdown of CUL4A inhibited the NF-κB pathway.

Notes: (A) HGC27 cells transfected with CUL4A siRNA were treated with 100 ng/mL of LPS for 2 h and analyzed for NF-κB protein levels by immunoblotting. (B) The expression of NF-κB downstream genes including VEGF, MMP2, MMP9, and IL-8 was analyzed by qRT-PCR. *P<0.05 and **P<0.01.

Abbreviations: CUL4A, cullin 4A; LPS, lipopolysaccharide; MMP, matrix metalloproteinase; IL-8, interleukin-8; NC, negative control; NF-κB, nuclear factor-kappa B; siRNA, short interfering RNA; qRT-PCR, quantitative real time polymerase chain reaction.