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. Author manuscript; available in PMC: 2018 Apr 11.
Published in final edited form as: Vaccine. 2017 Mar 18;35(16):2015–2024. doi: 10.1016/j.vaccine.2017.03.020

Figure 1. Schematic representations of DNA vaccine construct; analyses of its expression and experimental design in THY-Tau22 transgenic mice.

Figure 1

(A) Strategy for cloning the gene encoding 3Tau2-18-MultiTEP (AV-1980D) into the pVAX1 vector and schematic representation of AV-1980 construct encoding 3 copies of Tau2-18 fused to MultiTEP, one universal synthetic Th epitope, PADRE and eleven foreign promiscuous Th epitopes from infectious agents. (B) Intracellular and secreted AV-1980 protein from transfected CHO cells detected by WB, visualized by staining with anti-tau Mab, 1C9. (C) Design of experimental protocol in Tg mice vaccinated with AV-1980D and pMultiTEP (control group).