Skip to main content
. Author manuscript; available in PMC: 2018 Apr 11.
Published in final edited form as: Vaccine. 2017 Mar 18;35(16):2015–2024. doi: 10.1016/j.vaccine.2017.03.020

Figure 2. Cellular and humoral immune responses in THY-Tau22 mice vaccinated with AV-1980D.

Figure 2

(A) Numbers of IFN-γ producing T-cells were calculated by ELISPOT in splenocyte cultures obtained from vaccinated animals. Bars represent average ± SD (n=6 for pMultiTEP immunized group and n=7 for AV-1980D vaccinated group). (B) Concentration of anti-tau antibodies was detected in sera by ELISA. Lines indicate the mean values of antibody concentration (n=6). (C) Isotypes of anti-tau antibodies were analyzed by ELISA. Sera collected after two immunizations were used at dilution 1:800. (D) Binding to T helper epitopes incorporated in MultiTEP platform was detected in sera by ELISA. Sera collected after two immunizations were used at dilution 1:1000. (E) AV-1980D immune sera, but not pMultiTEP immune sera bound to the 50 μm brain sections of cortical tissues from two AD cases. Sera were used at dilution 1:1000. TNT-1 monoclonal antibody was used as a positive control. (F) AV-1980D immune sera, but not pMultiTEP immune sera bound to different forms of tau in soluble extracts from four AD cases detected by Western blot. Sera were used at dilution 1:1000. TNT-1 monoclonal antibody was used as a positive control.