Skip to main content
. Author manuscript; available in PMC: 2018 Feb 3.
Published in final edited form as: J Proteome Res. 2016 Dec 2;16(2):609–618. doi: 10.1021/acs.jproteome.6b00698

Table 1.

Main Instrument Parameters Used for Survey MS1 Scans during the Three Types of Data Acquisition Modes Used in This Studya

data acq. logic tech. rep.b resolving power (at 200 m/z) scan type scan range AGC target microscans max injection time
Top-2 hi/hid all 3 120 000 MS1 500–2000 m/z 1.00 × 1006 4 50 ms
AUTOPILOT hi/hid #1 120 000 MS1 and random SIM 500–2000 m/z MS1, 25 m/z SIMc 1e6 MS1, 5e4 SIM 4 MS1, 8 SIM 50 ms
#2 120 000 SIM march 4 × 50 m/z, 700–900 m/z 5.00 × 1004 8 400 ms
#3 120 000 SIM march 8 × 25 m/z, 700–900 m/z 5.00 × 1004 8
Top-2 med/hie all 3 3750 MS1 500–2000 m/z 1.00 × 1006 25 50 ms
a

Note the study design and use of technical replicates.

b

Technical replicates, where the same sample was subjected three times to LC–MS.

c

SIM, selected ion monitoring.

d

hi/hi refers to use of high-resolution data for both MS1 and MS2 scans.

e

med/hi (short hand for “medium/high”) refers to use of nonisotopically resolved data at a “medium” level of resolution for MS1 and high-resolution data for fragment ions recorded in a MS2 scan.