Table 2.
Bacterial strains and plasmids used in this study.
| Strains and plasmids | Genotype and relevant characteristics* | Reference or source |
|---|---|---|
| Strains | ||
| P. protegens | ||
| LK099 | Wild-type Pf-5 | (Howell and Stipanovic, 1979) |
| JL4928 | ΔpltAΔphlD | This study |
| LK269 | ΔpltAΔphlDΔpltR | This study |
| LK270 | ΔpltM | This study |
| LK024 | ΔphlD | (Kidarsa et al., 2011) |
| LK293 | ΔpltMΔpltR | This study |
| LK397 | ΔphlDΔpltM | This study |
| LK096 | ΔpltAΔphlA | (Henkels et al., 2014) |
| LK403 | ΔphlAΔpltM | This study |
| LK413 | ΔphlAΔphlDΔpltA | This study |
| P. fluorescens SBW25 | SBW25 is a member of the P. fluorescens group but does not contain the phl or plt gene clusters | (Silby et al., 2009) |
| E. coli | ||
| BL21(DE3) | ompT hsdSB (rB−mB−) gal dcm | NEB |
| S17-1 | recA pro hsdR-M+ RP4 2-Tc::Mu-Km::Tn7 Smr Tpr | (Simon et al., 1983) |
| Plasmids | ||
| pEX18Tc | Gene replacement vector with MCS from pUC18, sacB+ Tcr | (Hoang et al., 1998) |
| p18Tc-ΔpltM | pEX18Tc containing pltM with an internal deletion of 1345 bp | This study |
| pPROBE-NT | pBBR1 containing promoterless gfp, Kmr | (Miller et al., 2000) |
| pRL-gfp | pPROBE-NT containing pltR as well as the intergenic region between pltR and pltL including the promoter of pltL fused with a promoterless gfp | This study |
| pMRL-gfp | pPROBE-NT containing pltM and pltR as well as the intergenic region between pltR and pltL including the promoter of pltL fused with a promoterless gfp | This study |
| pL-gfp | Called ppltL-gfp previously. Contains the intergenic region between pltR and pltL including the promoter of pltL fused with a promoterless gfp | (Yan et al., 2016) |
| pME6010 | pACYC177-pVS1 shuttle vector, Tcr | (Heeb et al., 2000) |
| pME6010-pltM | pME6010 containing pltM, which is expressed from the constitutive promoter Pk | This study |
| pET28a(+) | Protein overexpression vector | Novagen |
| pET28a-PltM | pET28a containing pltM, used for overexpression of the PltM protein | This study |
| pET28a-SsuE | pET28a containing ssuE, used for overexpression of the SsuE protein | This study |
*Smr, streptomycin resistance; Tpr, trimethoprim resistance; Tcr, tetracycline resistance; Kmr, kanamycin resistance.