Table 3.
Sequences of oligonucleotides used in this study
| Oligonucleotides | DNA sequences* |
|---|---|
| pltR UpF-Xba | GAGAGGTCTAGAAGTGGAGTCTGGTCATCAAG |
| pltR UpR | GCTCTTGTCTTGTAGTCTTCCTGTTTCGGAGAA |
| pltR DnF | AACAGGAAGACTACAAGACAAGAGCCAGACATC |
| pltR DnR-Xba | GGTGTGTCTAGAGTGAAGAATGAGCAGGTGTC |
| pltM-F1 | ATGGTACCCTGCATTTCGATACCGC |
| pltM-R1 | ATAGAATTCGGCGGACGGGTGGATC |
| pltMRL-F1 | AGCGATTTCGATCTTCATCCCC |
| pltM-up-ovlp | GAAGCGGCGACACCGGATCCAATCGTTTCATCC |
| pltM-dn-ovlp | GGATGAAACGATTGGATCCGGTGTCGCCGCTTC |
| pltRf | ATGGTACCAAGGATTTAGGAATGAAGGC |
| pltM 5'primer | GTCATACATATGAATCAGTACGACGTCATTATC |
| pltM 3' primer | CAGTGCCTCGAGTCAGACTTTGAGGATGAAACG |
| ssuE 5' primer | GGAGAGCATATGCGTGTCATCACC |
| ssuE 3' primer | GTAAAGCTTTTACGCATGGGCATT |
| pltM 3' primer3 | ATAGGATCCGGCCCCGGGCATCACTCAG |
| pltRr | TATAAGCTTTTCAGCCCGGACTTCGCGAGG |
| gfp-plt-r1 | ATGGTACCATAGACGTACGCTCCTGC |
*Restriction sites used for cloning are underlined in oligonucleotides.