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. Author manuscript; available in PMC: 2017 Aug 27.
Published in final edited form as: Nat Struct Mol Biol. 2017 Feb 27;24(4):344–352. doi: 10.1038/nsmb.3384

Figure 2. Active TOP2 is recruited immediately upon BAF recruitment and is required for the initial stage of accessibility induction.

Figure 2

(a) Strategy for timecourse of active TOP2/strand-cleaved decatenation intermediate detection during BAF recruitment. (b) TOP2A etoposide-ChIP in cells treated with 3 nM rapamycin for various times before fixation with 100 μM etoposide. (c) Strategy for timecourse of TOP2 inhibition during BAF recruitment. (d) ATAC-qPCR in cells treated with rapamycin and 1 μM ICRF-193 after various delays. Significance assessed by t-tests as before. Bars represent means and error bars represent s.e.m. from 6 (b) or 3 (d) cell passages.