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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: J Bone Miner Res. 2017 Feb 27;32(4):821–833. doi: 10.1002/jbmr.3045

Fig. 5.

Fig. 5

Fluoxetine inhibits osteogenic differentiation of bone marrow stromal cell in vitro. (A) TUNEL assay of BMSCs in GM with varying concentrations of fluoxetine. (B) Proliferation assay using BrdU labeling of BMSC in growth media with varying concentrations of fluoxetine. (C) Alizarin red staining was quantified using absorbance, representative images of the mineralization assay are shown below. (D) ALP activity of BMSCs in OM and varying concentrations of fluoxetine. (E) Gene expression of col 1, oc, and ALP in BMSC cultured in OM and fluoxetine. Asterisk denotes statistical significance (p < 0.05), statistical comparison against growth/osteogenic media control. alp = alkaline phosphatase; BrdU = Bromodeoxyuridine; col 1 = collagen type 1; f = fluoxetine; GM = growth media; oc = osteocalcin; OM = osteogenic differentiation media; BMSC = bone marrow stromal cell.