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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Animal. 2016 Oct 20;11(5):811–819. doi: 10.1017/S1751731116002044

Figure 4.

Figure 4

Effect of angiogenin (ANG) on steroidogenic enzyme gene expression in granulosa and theca cells (experiment 7). (a) Effect of effect of ANG (300 ng/ml) on CYP19A1 (aromatase) and CYP11A1 (side-chain cleavage enzyme) mRNA abundance in small follicle granulosa cells. (b) Effect of ANG (300 ng/ml) on CYP17A1 and CYP11A1 mRNA abundance in large follicle theca cells. Granulosa cells from small follicles and theca cells from large follicles were cultured for 48 h in the presence of 10% FCS, and then treated in serum-free medium with ANG for 24 h. Granulosa cells were also concomitantly treated with 30 ng/ml of IGF1 and 30 ng/ml of FSH, and theca cells were concomitantly treated with 30 ng/ml of IGF1 and 30 ng/ml of LH. Values are means of three separate experiments (± SEM) and normalized to constitutively expressed 18S ribosomal RNA. *Mean differs (P <0.05) from its respective control (0 ng/ml of ANG).