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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Graefes Arch Clin Exp Ophthalmol. 2017 Jan 25;255(5):955–962. doi: 10.1007/s00417-017-3594-0

Figure 3.

Figure 3

DNA methylation machinery is modulated by oxidative stress: The activities of (a) Dnmt1 and (b) Tet, and mRNA levels of (c) MMP-9 and (d) mtDNA-encoded Cytb were quantified in retinal endothelial cells incubated in 5mM or 20mM glucose for 4 days in the presence or absence of 250μM MnTBAP. The enzyme activities and mRNA values obtained from the cells incubated in 5mM glucose alone are considered as 100% and 1 respectively. Values are represented as mean ± SD from 3–5 samples in each group, with each measurement made in duplicate. 5mM and 20mM=cells incubated in 5mM and or 20mM glucose respectively; 20+Mn= cells in 20mM glucose in the presence of MnTBAP; Mann=cells in 20mM mannitol. *p< 0.05 compared to 5mM glucose.