Fig. 4.

Effect of MRBH on VEGF-induced cell migration.

VEGF-induced migration of HUVECs was examined in a wound-healing assay. After the wounded HUVECs were treated with vehicle (control), VEGF (10 ng/ml), VEGF plus MRBH (0.3, 1, or 3 mg/ml), or MRBH alone (0.3, 1, or 3 mg/ml) for 21 hr, images were taken (A), and the number of cells migrating into the wounded region was counted (B). Scale bars, 100 μm. Data in B are presented as the mean ± SEM (n=4). Means not sharing a common letter are significantly different (p<0.01).