Fig. 5.

Effect of MRBH on VEGF-induced phosphorylation of VEGFR2 and downstream signaling proteins.

HUVECs were pretreated with vehicle or MRBH (3 mg/ml) for 30 min and then treated with or without VEGF (10 ng/ml) for 15 min. Phosphorylation of VEGFR2, Akt, ERK1/2, and P38 in whole cell lysates was determined by western blot analysis. (A) Data are representative of three individual experiments with identical conditions. (B–E) Band intensities were quantified and represented as a bar graph. Data are presented as the mean ± SEM (n=3). Means not sharing a common letter are significantly different (p<0.05 for C and D; p<0.01 for B and E).