Fig. 2. Identification of MAT idiomorph in single spores isolated from Cordyceps militaris. A, Conidia suspension from mycelium was smeared and single spore marked circle was germinated in water agar (WA) plate after 5 days; B, Stroma with perithecia was attached to WA plate cover and single spores marked circle was germinated in WA plate after 5 days; C, MAT1-1-1 partial gene of MAT1-1 and MAT1-2-1 partial gene of MAT1-2 was amplified. The PCR products were electrophoresed on a 1.5% agarose gel. M, 100 bp DNA ladder molecular size marker. Positive control and negative control used single spores which MAT idiomorph were known by sequencing. MAT1-1-1 was successfully amplified in MAT1-1 idiomorph seven single spores. MAT1-1-1 was not amplified in MAT1-2 idiomorph five single spores; D, MAT1-2-1 was successfully amplified in MAT1-2 idiomorph five single spores. MAT1-2-1 was not amplified in MAT1-1 idiomorph seven single spores.