Figure 2.

Calpain and calpastatin expression in control animals and in mice implanted with the C26 tumor. Calpain protein levels in the tibialis muscle (A) and in the heart (B) of controls and C26 hosts. (C) Calpastatin digestion products were detected in the tibialis muscle of controls and C26 tumor-bearing mice by the mAb 35,23. (D) Immunofluorescence images of tibialis muscle sections in controls and C26-bearing mice stained for calpastatin (green) and nuclei (blue). Densitometric quantifications (histograms) were normalized according to tubulin levels. Blot representative patterns are shown. Data (mean ± SD, n = 6) are expressed as % of controls. Significance of the differences (t-test): ^***p < 0.001 vs. controls.