Figure 3.

Real-time PCR quantification of the differentially expressed microRNAs and their putative target transcription factors from infected and control plants at 24 hpi. (A) Expression of differentially expressed miRNAs in ALT1-inoculated and control Golden Delicious (GD) apple leaves. (B) Expression of corresponding miRNA target genes in ALT1-inoculated and control GD leaves. Spore inoculum concentration: 2 × 10⁵ CFU/ml. Spore growth was measured at 6 dpi. Real-time PCR data were calculated based on three biological and three technical replicates. Error bars = SD; ^**P < 0.01; ^*P < 0.05 (Student's t-test).