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. 2017 Apr 19;8:519. doi: 10.3389/fpls.2017.00519

Figure 7.

Figure 7

Estradiol-induced overexpression of ERD10 in BY-2 cells and Arabidopsis thaliana cell suspension. ERD10 gets overexpressed in transformed BY-2 cells upon induction with estradiol as shown by a dual color simultaneous western blotting technique. Recombinantly produced and purified ERD10 was included as a reference for the molecular weight of the native protein without any kind of post-translational modification (std ERD10). Expression of ERD10 was monitored from to (the point in which estradiol was added) until 86 h. The endogenous tubulin was monitored as a reference to the cell WT refers to samples from non-modified plant cells treated identically as genetically modified plant cells that contain erd10 under control of the inducible promoter.