Table 1.

Scouting of various electroporation conditions.

	Experimental condition
Waveform	1	2	3	4	5	6	7	8	9	10	11	12
Exponential	150 V	200 V	250 V	300 V	350 V	450 V	250 V	250 V	250 V	250 V	250 V	250 V
decay	350 μF	350 μF	350 μF	350 μF	350 μF	350 μF	200 μF	250 μF	350 μF	500 μF	750 μF	1,000 μF
	400 V	250 V	150 V	100 V	50 V	50 V	150 V	150 V	150 V	150 V	50 V	50 V
	200 μF	200 μF	200 μF	200 μF	200 μF	100 μF	250 μF	500 μF	750 μF	1,000 μF	500 μF	750 μF
Square	150 V	200 V	250 V	300 V	350 V	450 V	250 V	250 V	250 V	250 V	250 V	250 V
wave	20 ms	20 ms	20 ms	20 ms	20 ms	20 ms	5 ms	10 ms	15 ms	20 ms	25 ms	30 ms

The electric pulse is a wave with an associated voltage, capacitance and time. There are two different waveforms, namely square wave or exponential decay. These experimental conditions were tested to load BY-2 cells with externally supplied ERD14-C186-Alexa Fluor 647 as reporter protein. We monitored the cell integrity and cell viability as a biological read-out through microscopic observations.