FIG 3 .

DNA binding activities of RctB mutants with single-residue changes in the DnaK interaction site (K-I site). (A) DNA binding by EMSA. The RctB mutants were R154L, L155R, L156R, H157E, and L161R. Other details are same as those described for Fig. 2A. (B) DNA binding of RctB in vivo. The RctB proteins were same as those described for panel A but were left untagged; otherwise, the details are same as those described for Fig. 2B. (C) Initiator activity of the RctB in vivo. RctB sources were same as those described for panel B; otherwise, the details are same as those described for Fig. 1E.