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Phase contrast microscopy. (A) Controls showing untreated MDA-MB-231 human breast cancer cells. Cytotoxic activity of ethyl acetate vapor saturated flasks at (B) 0.02 M, (C) 0.026 M, (D) 0.03 M, (E) 0.034 M, (F) 0.04 M, (G) 0.05 M, (H) 0.1 M, (I) 0.2 M, and (J) 0.5 M on MDA-MB-231 cells after 48 hours (magnification 10×).
