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EA induced necrosis of human breast cancer cell line MDA-MB-231, visualized with acridine orange–ethidium bromide assay. (A) Cells from control flask; cells in presence of (C) 0.026 M (E) 0.1 M, and (G) 0.2 M EA after 48 hours (10× magnification) (B, D, F, H) same at 40× magnification, respectively. Uniformly stained green-colored nuclei indicate live cells; whereas uniformly stained orange–red-colored cells are indicative of necrosis.
EA, ethyl acetate.
