Figure 6.

Depletion of Rab5a impairs nuclear lamina disassembly in oocytes. A) Control and Rab5a-MO oocytes were labeled with anti-lamin A/C antibody (green) and counterstained with PI to visualize chromosomes (red). Representative images of GV and MI oocytes are shown. Arrowhead in Rab5a-MO MI oocyte shows residual nuclear lamina as judged by lamin A/C staining. Scale bar = 25 μm. B) Quantification of control and Rab5a-MO oocytes showing persistent lamin at metaphase stage. At least 50 oocytes were counted for each group. Bars represent means ± sd. *P < 0.05 vs. controls.