Figure 5.

Female offspring of HFD-treated dams presented significantly increased BW and leptin levels from weaning to adulthood. Second-generation offspring of either C- or HFD-fed dams were euthanized at weaning day (pups: C or HFD; A–C) or were raised till PND 80 (adults: C-C or HFD-C; D–F) on C diet. A, D) Average BW (n=10/group). B, E) Plasma leptin levels (pups: n=7–8/group; adults: n=6–8/group). C, F) mRNA levels of Pomc (pups: n=8–11/group; adults: n=5–6/group). Relative gene expression in the C group was set to 1. Data are presented as means ± sem. *P < 0.05 vs. C.