Table 1.
Percent prevalence of dhfr alleles at codons 51, 59 and 108 from isolates collected in Senegal and Tanzania using nested polymerase chain reaction/restriction fragment length polymerase (PCR/RFLP) and high resolution melting (HRM)
| Senegal (N: 50) | Tanzania (N: 50) | ||||||
|---|---|---|---|---|---|---|---|
| PCR/RFLP | HRM | p value | PCR/RFLP | HRM | p value | ||
| DHFR 51 | N51 | 1/50 (2%) | 0 | 1 | 09/50 (18%) | 08/50 (16%) | 1 |
| 51I | 49/50 (98%) | 47/50 (94%) | 0.6173 | 37/50 (74%) | 26/50 (52%) | 0.0365 | |
| N51 + 51I | 0 | 3/50 (6%) | 0.2424 | 04/50 (08%) | 16/50 (32%) | 0.005 | |
| DHFR 59 | C59 | 2/50 (4%) | 1/50 (2%) | 1 | 19/50 (38%) | 12/50 (24%) | 0.1941 |
| 59R | 48/50 (96%) | 46/50 (92%) | 0.6777 | 24/50 (48%) | 22/50 (44%) | 0.8411 | |
| C59 + 59R | 0 | 3/50 (6%) | 0.2424 | 07/50 (14%) | 16/50 (32%) | 0.0558 | |
| DHFR 108 | S108 | 2/50 (4%) | 0 | 0.4949 | 07/50 (14%) | 07/50 (14%) | 1 |
| 108N | 48/50 (96%) | 49/50 (98%) | 1 | 35/50 (70%) | 38/50 (76%) | 0.6528 | |
| S108 + 108N | 0 | 1/50 (2%) | 1 | 08/50 (16%) | 05/50 (10%) | 0.5535 | |
N total number of patient