Table 3.
Prevalence of mutations in dhfr and dhps in Senegal and Tanzania when accounting for number of parasite genomes per sample
| Alleles | Parasite genome | |||
|---|---|---|---|---|
| Senegal | Tanzania | p value | ||
| DHPS 437 | A437 | 27/80 (33.75%) | 42/103 (40.78%) | 0.9729 |
| G437 | 45/80 (56.25%) | 39/103 (37.86%) | 0.0131 | |
| Undetermined | 8/80 (10%) | 22/103 (21.36%) | ||
| DHPS 540 | K540 | 80/80 (100%) | 103/103 (100%) | >0.05 |
| E540 | 0/80 (0%) | 0/103 (0%) | >0.05 | |
| Undetermined | 0/80 (0%) | 0/103 (0%) | ||
| DHPS 581 | A581 | 80/80 (100%) | 103/103 (100%) | >0.05 |
| G581 | 0/80 (0%) | 0/103 (0%) | >0.05 | |
| Undetermined | 0/80 (0%) | 0/103 (0%) | ||
| DHPS 613 | A613 | 75/80 (3.75%) | 100/103 (97.09%) | 0.2713 |
| T/S613 | 2/80 (2.5%) | 0/103 (0%) | 0.1074 | |
| Undetermined | 3/80 (3.75%) | 3/103 (2.91%) | ||
| DHFR 51 | N51 | 0/80 (0%) | 21/103 (20.39%) | 0 |
| 51I | 77/80 (96.25%) | 67/103 (65.05%) | 0 | |
| Undetermined | 3/80 (3.75%) | 15/103 (14.56%) | ||
| DHFR 59 | C59 | 1/80 (1.25%) | 32/103 (31.07%) | 0 |
| 59R | 76/80 (95%) | 56/103 (54.37%) | 0 | |
| Undetermined | 3/80 (3.75%) | 15/103 (14.56%) | ||
| DHFR 108 | S108 | 0/80 (0%) | 16/103 (15.5%) | 0.0002 |
| N108 | 79/80 (98.75%) | 87/103 (84.5%) | 0.0096 | |
| Undetermined | 1/80 (1.25%) | 0/103 (0%) | ||
| Polyclonal infections | 27/50 (54%) | 36/50 (72%) | 0.0628 | |
| Multiplicity of infection | 78/50 (1.56) | 103/50 (2.06) | 0.011 | |
The number of parasite genotypes per patient was calculated to estimate the wild-type and mutant allele frequencies in mixed infections. Undetermined represents samples in which the number of genotypes cannot be precisely classified due to uncertainty (for example, if there are 4 genomes, the call could be 1 WT and 3 Mut, 2 WT and 2 Mut, or 3 WT and 1 Mut)