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. 2017 Apr 18;8:90. doi: 10.1186/s13287-017-0542-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Flow cytometry analysis, cytokine proteins and mRNA measurements of Th1 cells from PBMCs in the AHSCT and Insulin-only groups before and after treatment (0 M and 12 M). a, b Representative flow cytometry plots of CD3⁺CD4⁺ (R2) and Th1 cells (R20). c Proportion of IFN-γ⁺ Th1 cells in PBMCs. d, e, f mRNA expression levels of IL-2, IL-12p40 and T-bet respectively. g, h, j Concentrations of IL-2, IL-12p40 and IFN-γ in the cell culture supernatants respectively. *P < 0.05, **P < 0.01, ***P < 0.001. AHSCT autologous hematopoietic stem cell transplantation, ns no significance, PBMC peripheral blood mononuclear cell