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. 2017 Apr 18;8:80. doi: 10.1186/s13287-017-0525-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Immunohistochemistry of pericyte, adventitial cell, and MSC markers in equine tissues. a–c Pericytes stained for CD146 surrounding CD144⁺ endothelial cells in adipose tissue (a), testis (b), and skeletal muscle (c). d, e Dual staining (right panel) with the pericyte markers NG2 and CD146 (d) or NG2 and αSMA (e) in adipose tissue and testis, respectively. f, g Colocalization (right panel) of the MSC and pericyte markers CD29 and NG2 (f) and CD44 and CD146 (g) in adipose tissue, respectively. d–g Individual staining is shown (left and middle panels). h, i Immunodetection of the adventitial cell marker CD34 (h) and the MSC marker CD44 (i) in the outer layer of blood vessels (arrows) in adipose tissue. 4′,6-Diamidino-2-phenylindole (DAPI) was used to stain cell nuclei. White scale bars = 10 μm