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Gene expression analysis of non-sorted, CD34+, and CD146+ cultured cells. mRNA levels were quantified by qPCR using primers for a CD146 (pericyte marker), b CD34 (adventitial cell), and c CD144 (endothelial cells) in non-sorted (light gray), CD34+ (dark gray) and CD146+ (black) cells at passage 1 or 2 (P1/2), 3 or 4 (P3/4), and 7 or 8 (P7/8). n = 3–6; mean ± SEM. *p < 0.05, **p < 0.001 between means. AU arbitrary units, ND not detected
